Bethyl Laboratories
Rabbit anti-c-Maf Recombinant Monoclonal Antibody [BLR045F]
Monoclonal Antibodies
Catalog #A700-045
Targetc-Maf
HostRabbit
CloneBLR045F
ReactivityMouse, Human
ApplicationICC, IHC, IP, WB
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Product Description
c-Maf is part of the Maf family of transcription factors that possess a conserved basic region and a leucine zipper domain that mediate DNA and protein-protein binding and plays an important role in tissue-specific gene regulation and cell differentiation. c-Maf is a transcriptional activator of interleukin 4 and 10 in T-cells, and is involved in lens fiber cell differentiation. c-Maf translocation has been observed in human multiple myeloma and may be oncogenic.
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR045F
Host
Rabbit
Immunogen
Between 125 and 175
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
Targetc-Maf
Antigen Species
Human
Gene ID4094
Gene Symbol
MAF
Gene Name
MAF bZIP transcription factor
Uniprot IDO75444
Protein Name
Transcription factor Maf
Alternate Names
Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene • AYGRP • CCA4 • c-MAF • c-maf proto-oncogene • CTRCT21 • proto-oncogene c-Maf • T lymphocyte c-maf long form • transcription factor Maf • v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog • V-maf musculoaponeurotic fibrosarcoma oncogene homolog
Product Information
Applications
ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residues 125 and 175 of human MAF bZIP transcription factor (c-Maf) using the numbering given in entry NP_005351.2 (Gene ID 4094).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-045 · Lot 3 (current lot)Open
Product DatasheetPDF · A700-045 · Lot 3 (current lot)Open
Safety Data SheetPDF · A700-045Open
Citations / Publications
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Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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