Bethyl Laboratories
Rabbit anti-CBX3 Antibody Affinity Purified
Primary
Catalog #A300-984A
TargetCBX3
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP
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Product Description
CBX3 (chromobox protein homolog 3), also known as HP1gamma (heterochromatin protein 1 homolog gamma), is one of 3 isoforms of HP1 that binds heterochromatin and functions as a structural adaptor to stabilize and organize macromolecular complexes with nucleosomes for higher chromatin structure. All of the HP1 isoforms possess a chromodomain and chromoshadow domain and associate with methylated histones that mediate transcriptional repression.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 133 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCBX3
Antigen Species
Human
Gene ID11335
Gene Symbol
CBX3
Gene Name
chromobox 3
Uniprot IDQ13185
Protein Name
Chromobox protein homolog 3
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CBX3 immobilized on solid support.
The epitope recognized by A300-984A maps to a region between residue 133 and the C-terminus (residue 183) of human chromobox homolog 3 using the numbering given in entry NP_009207.2 (GeneID 11335).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:2000 - 10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A300-984A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-984A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-984AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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