Bethyl Laboratories
Rabbit anti-CD137 Recombinant Monoclonal Antibody [BLR051F]
Monoclonal Antibodies
Catalog #A700-051
TargetCD137
HostRabbit
CloneBLR051F
ReactivityHuman
ApplicationFlow Cyt, ICC, IHC, IP, WB
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Product Description
CD137 is a member of the TNF-receptor superfamily; it is a receptor that contributes to the clonal expansion, survival, and development of T cells. CD137 can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. [provided by RefSeq, Jul 2008] [taken from NCBI Entrez Gene (Gene ID: 3604)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR051F
Host
Rabbit
Immunogen
Residues 24-189 (ECD)
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetCD137
Antigen Species
Human
Gene ID3604
Gene Symbol
TNFRSF9
Gene Name
TNF receptor superfamily member 9
Uniprot IDQ07011
Protein Name
Tumor necrosis factor receptor superfamily member 9
Alternate Names
4-1BB • 4-1BB ligand receptor • CD antigen CD137 • CD137 • CD137 antigen • CDw137 • homolog of mouse 4-1BB • ILA • induced by lymphocyte activation (ILA) • interleukin-activated receptor, homolog of mouse Ly63 • receptor protein 4-1BB • T cell antigen ILA • T-cell antigen 4-1BB homolog • T-cell antigen ILA • tumor necrosis factor receptor superfamily member 9
Product Information
Applications
Flow Cyt, ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein representing the extracellular domain of human CD137 (residues 24-189, NP_001552.2).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-051 · Lot 4 (current lot)Open
Product DatasheetPDF · A700-051 · Lot 4 (current lot)Open
Safety Data SheetPDF · A700-051Open
Citations / Publications
Services
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