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Rabbit anti-CDT1 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog # A300-786A

Validated
Target:
CDT1
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 500 and C-term
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Human
Antigen Species
Human
Stock Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Request Carrier-free Quote
Prod Procedures
Antibody was affinity purified using an epitope specific to CDT1 immobilized on solid support.

The epitope recognized by A300-786A maps to a region between residue 500 and the C-terminus (residue 546) of human Chromatin Licensing and DNA Replication Factor 1 using the numbering given in entry NP_112190.1 (GeneID 81620).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

CDT1 is involved in the formation of pre-replicative complexes (preRC) at origins of replication. Under tight regulation, CDT1 associates with chromatin during G1 in a dynamic manner to promote the loading of mini-chromosome maintenance (MCM) complex in preparation for replication. CDT1 is negatively regulated by ubiquitin-directed degradation and has been demonstrated to be protected from ubiquitination by the protein geminin.

81620

CDT1

chromatin licensing and DNA replication factor 1

Q9H211

DNA replication factor Cdt1

Alternate Names

DNA replication factor Cdt1, Double parked homolog, Double parked, Drosophila, homolog of, DUP, RIS2

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-20% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Citations (1)