Bethyl Laboratories
Rabbit anti-CEACAM1/5 Recombinant Monoclonal Antibody [BLR032F]
Monoclonal Antibodies
Catalog #A700-032
TargetCEACAM1/5
HostRabbit
CloneBLR032F
ReactivityHuman
ApplicationFlow Cyt, ICC, IHC, IP, WB
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Product Description
Carcinoembryonic antigen related cell adhesion molecules 1 and 5 (CEACAM1 and CEACAM5) are members of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. CEACAM1 was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. CEACAM5 is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, CEACAM5 may regulate differentiation, apoptosis, and cell polarity.
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR032F
Host
Rabbit
Immunogen
Residues 35-433 (ECD)
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
50 µg/ml
Target Identity
TargetCEACAM1/5
Antigen Species
Human
Gene ID634
Gene Symbol
CEACAM1
Gene Name
carcinoembryonic antigen related cell adhesion molecule 1
Uniprot IDP13688
Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 1
Product Information
Applications
Flow Cyt, ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein representing the extracellular domain of human CEACAM1 (residues 35-433, NP_001020083.1).
By western blot of transfected cells expressing CEACAM1 and transfected cells expressing CEACAM5, this antibody was confirmed to bind both CEACAM1 and CEACAM5.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-032 · Lot 2 (current lot)Open
Product DatasheetPDF · A700-032 · Lot 2 (current lot)Open
Product DatasheetPDF · A700-032 · Lot 2 (current lot)Open
Safety Data SheetPDF · A700-032Open
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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