Bethyl Laboratories
Rabbit anti-CEACAM5 Recombinant Monoclonal Antibody [BLR198J]
Monoclonal Antibodies
Catalog #A700-198
TargetCEACAM5
HostRabbit
CloneBLR198J
ReactivityHuman
ApplicationICC, IHC, IP, WB
Image 1 of 4
local_shipping
Order within 20 hours and 33 minutes for shipment by Tuesday, November 17
Product Description
Carcinoembryonic antigen related cell adhesion molecules 1 and 5 (CEACAM1 and CEACAM5) are members of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. CEACAM1 was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. CEACAM5 is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, CEACAM5 may regulate differentiation, apoptosis, and cell polarity.
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR198J
Host
Rabbit
Immunogen
Residues 35-685 (ECD)
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
100 µg/ml
Target Identity
TargetCEACAM5
Antigen Species
Human
Gene ID1048
Gene Symbol
CEACAM5
Gene Name
carcinoembryonic antigen related cell adhesion molecule 5
Uniprot IDP06731
Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 5
Product Information
Applications
ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein representing the extracellular domain of human Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5, residues 35-685, NP_001278413.1)
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A700-198 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-198 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-198 · Lot 210719Open
Safety Data SheetPDF · A700-198Open
Related Products
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request Customization
Custom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal Development
VHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH Discovery
Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
Explore Simple Western™
1