Bethyl Laboratories
Rabbit anti-CEACAM8/CD66b Recombinant Monoclonal Antibody [BLR111H]
Monoclonal Antibodies
Catalog #A700-111
TargetCEACAM8/CD66b
HostRabbit
CloneBLR111H
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
CEACAM8/CD66b member of the CEACAM subfamily of the CEA family of proteins. It is a cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner. It is expressed by neutrophils and eosinophils, and mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P31997].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR111H
Host
Rabbit
Immunogen
Residues 23-320 (ECD)
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
250 µg/ml
Target Identity
TargetCEACAM8/CD66b
Antigen Species
Human
Gene ID1088
Gene Symbol
CEACAM8
Gene Name
carcinoembryonic antigen related cell adhesion molecule 8
Uniprot IDP31997
Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 8
Product Information
Applications
IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein representing the extracellular domain of human carcinoembryonic antigen-related cell adhesion molecule 8 (aka CD66b, residues 23-320, NP_001807.2).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-111 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-111 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-111 · Lot 200203Open
Safety Data SheetPDF · A700-111Open
Services
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