Bethyl Laboratories
Rabbit anti-DBC1/p30 DBC IHC Antibody Affinity Purified
Primary
Catalog #IHC-00135
TargetDBC1/p30 DBC
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
The DBC1 (Deleted in Breast Cancer Gene 1 protein) gene is located in a region frequently deleted in breast cancers. In its uncleaved form, DBC1 is localized to the nucleus. Upon cleavage by caspase activity, DBC1 becomes localized to the cytoplasm and functions to promote apoptosis. DBC1 has also been found to bind directly to the estrogen receptor alpha (ERalpha) hormone binding domain to modulate ERalpha expression and mediate hormone-independent breast cancer cell survival.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
50 µg/ml
Target Identity
TargetDBC1/p30 DBC
Antigen Species
Human
Gene ID57805
Gene Symbol
CCAR2
Gene Name
cell cycle and apoptosis regulator 2
Uniprot IDQ8N163
Protein Name
Cell cycle and apoptosis regulator protein 2
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DBC1/p30 DBC immobilized on solid support.
The epitope recognized by IHC-00135 maps to a region between residues 1 and 50 of human Deleted in Breast Cancer 1 using the numbering given in TrEMBL entry Q6P0Q9 (GeneID 57805).
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00135Open
Product DatasheetPDF · IHC-00135 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00135 · Lot 1 (current lot)Open
Citations / Publications
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Resources
Standard Western Blot Protocol
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