Bethyl Laboratories
Rabbit anti-DDB1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00134
TargetDDB1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
DDB1 (DNA Damage Binding Protein 1) is a subunit of the DNA damage-binding protein complex (DDB) involved in DNA repair. As part of a complex with Cul4-based ubiquitin ligase, Cul4-Ddb1 can directly modify chromatin through the ubiquitination of histones as well as ubiquitinate other chromatin bound factors. A deficiency in Cul4-Ddb1 ubiquitin ligase has been associated with xeroderma pigmentosum, a heritable disease characterized by defects in nucleotide excision repair and skin cancer development.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1090 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
50 µg/ml
Target Identity
TargetDDB1
Antigen Species
Human
Gene ID1642
Gene Symbol
DDB1
Gene Name
damage specific DNA binding protein 1
Uniprot IDQ16531
Protein Name
DNA damage-binding protein 1
Alternate Names
damage-specific DNA binding protein 1, 127kDa • Damage-specific DNA-binding protein 1 • DDB p127 subunit • DDBA • DNA damage-binding protein 1 • DNA damage-binding protein a • HBV X-associated protein 1 • UV-damaged DNA-binding factor • UV-damaged DNA-binding protein 1 • UV-DDB 1 • UV-DDB1 • XAP1 • XAP-1 • xeroderma pigmentosum group E-complementing protein • XPCE • XPE • XPE-BF • XPE-binding factor
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DDB1 immobilized on solid support.
The epitope recognized by IHC-00134 maps to a region between residues 1100 and the C-terminus (residue 1140) of human damage-specific DNA binding protein 1 using the numbering given in TrEMBL entry Q13289 (GeneID 1642).
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00134Open
Product DatasheetPDF · IHC-00134 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00134 · Lot 1 (current lot)Open
Citations / Publications
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Resources
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