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Rabbit anti-IDH3B Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
IDH3B
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 75 and 125
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

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Product Details

Specifications
Verified Reactivity
Mouse, Human
Presumed Reactivity
Bovine, Orangutan
Antigen Species
Human
Stock Concentration
100 µg/ml
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to IDH3B immobilized on solid support.

The epitope recognized by A305-501A-T maps to a region between residue 75 to 125 of human Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial using the numbering given in entry O43837.2 (GeneID 3420).

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Gene & Protein Information

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit.Isocitric dehydrogenase subunit beta (IDH3B) is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase [taken from NCBI Entrez Gene (Gene ID: 3420)].

3420

IDH3B

isocitrate dehydrogenase 3 (NAD(+)) beta

O43837

Isocitrate dehydrogenase [NAD] subunit beta, mitoc

Alternate Names

isocitrate dehydrogenase NAD subunit beta, mitochondrial, isocitrate dehydrogenase 3 (NAD+) beta, isocitric dehydrogenase subunit beta, NAD(+)-specific ICDH subunit beta, RP46

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-12% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-12% SDS-PAGE.