Bethyl Laboratories

Rabbit anti-DDX27 Antibody Affinity Purified

Primary
Catalog #A302-216A
TargetDDX27
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP
Detection of human DDX27 by western blot of immunoprecipitates.
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Product Description

DDX27 (DEAD-box protein 27) is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. The function of DDX27 has not been characterized.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 700 and 750
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDDX27
Antigen Species
Human
Gene ID55661
Gene Symbol
DDX27
Gene Name
DEAD-box helicase 27
Uniprot IDQ96GQ7
Protein Name
Probable ATP-dependent RNA helicase DDX27
Alternate Names
DEAD (Asp-Glu-Ala-Asp) box polypeptide 27  •  DEAD box protein 27  •  DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 27  •  Deficiency of ribosomal subunits protein 1 homolog  •  dJ686N3.1  •  DRS1  •  Drs1p  •  HSPC259  •  PP3241  •  probable ATP-dependent RNA helicase DDX27  •  RHLP  •  RNA helicase-like protein
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to DDX27 immobilized on solid support.

The epitope recognized by A302-216A maps to a region between residue 700 and 750 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 using the numbering given in entry NP_060365.6 (GeneID 55661).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate

Documents

Product DatasheetPDF · A302-216A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-216A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-216AOpen

Citations / Publications

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