Bethyl Laboratories
Rabbit anti-DDX27 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00634
TargetDDX27
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IHC-IF
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Product Description
DDX27 (DEAD-box protein 27) is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. The function of DDX27 has not been characterized.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 700 and 750
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetDDX27
Antigen Species
Human
Gene ID55661
Gene Symbol
DDX27
Gene Name
DEAD-box helicase 27
Uniprot IDQ96GQ7
Protein Name
Probable ATP-dependent RNA helicase DDX27
Alternate Names
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DDX27 immobilized on solid support.
The epitope recognized by IHC-00634 maps to a region between residue 700 and 750 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 using the numbering given in entry NP_060365.6 (GeneID 55661).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00634Open
Product DatasheetPDF · IHC-00634 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00634 · Lot 1 (current lot)Open
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