Bethyl Laboratories

Rabbit anti-DDX6 IHC Antibody Affinity Purified

Primary
Catalog #IHC-00430
TargetDDX6
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
Detection of human DDX6 by immunohistochemistry.
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Product Description

DDX6 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX6 has been observed to positively regulate growth by modulating gene expression at the translational level.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetDDX6
Antigen Species
Human
Gene ID1656
Gene Symbol
DDX6
Gene Name
DEAD-box helicase 6
Uniprot IDP26196
Protein Name
Probable ATP-dependent RNA helicase DDX6
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to DDX6 immobilized on solid support.

The epitope recognized by IHC-00430 maps to a region between residues 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 using the numbering given Swiss-Prot entry P26196 (GeneID 1656).

Applications

Epitope exposure is recommended.

Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.

Immunohistochemistry (IHC)1:100 - 1:500

Documents

Safety Data SheetPDF · IHC-00430Open
Product DatasheetPDF · IHC-00430 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00430 · Lot 1 (current lot)Open

Citations / Publications

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