Bethyl Laboratories
Rabbit anti-DNAM-1/CD226 Recombinant Monoclonal Antibody [BLR063G]
Monoclonal Antibodies
Catalog #A700-063
TargetDNAM-1/CD226
HostRabbit
CloneBLR063G
ReactivityHuman
ApplicationICC, IHC, IP, WB
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Product Description
DNAX accessory molecule-1 (DNAM-1) is a receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15762].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR063G
Host
Rabbit
Immunogen
Residues 19-229 (ECD)
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetDNAM-1/CD226
Antigen Species
Human
Gene ID10666
Gene Symbol
CD226
Gene Name
CD226 molecule
Uniprot IDQ15762
Protein Name
CD226 antigen
Product Information
Applications
ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein representing the extracellular domain of human DNAM-1/CD226 (residues 19-229, NP_006557.2).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-063 · Lot 3 (current lot)Open
Product DatasheetPDF · A700-063 · Lot 3 (current lot)Open
Safety Data SheetPDF · A700-063Open
Citations / Publications
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Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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