Bethyl Laboratories

Rabbit anti-GANP Antibody Affinity Purified

Primary
Catalog #A303-128A
TargetGANP
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
Detection of human GANP by western blot.
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Product Description

Germinal center –associated nuclear protein (GANP) has domains for MCM3-binding and RNA-primase activities. GANP has recently been reported to be transcribed from the same locus as MCM3AP.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1930 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetGANP
Antigen Species
Human
Gene ID8888
Gene Symbol
MCM3AP
Gene Name
minichromosome maintenance complex component 3 associated protein
Uniprot IDO60318
Protein Name
Germinal-center associated nuclear protein
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to GANP immobilized on solid support.

The epitope recognized by A303-128A maps to a region between residue 1930 and 1980 of human Germinal center-Associated Nuclear Protein using the numbering given in entry NP_003897.2 (GeneID 8888).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western Blot (WB)1:1000 - 1:5000 (3-8% Tris-acetate gel recommended)

Documents

Product DatasheetPDF · A303-128A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-128A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-128AOpen

Citations / Publications

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