Bethyl Laboratories
Rabbit anti-Phospho MEK1(T286) Antibody Affinity Purified
Primary
Catalog #A303-610A
TargetMEK1 Phospho (T286)
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP
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Product Description
MEK1 is an activator and kinase for MAP and ERK kinases. It is a serine/threonine kinase essential to the MAP kinase signal transduction pathway which is involved in a multitude of cellular processes such as proliferation, differentiation, gene activation, and development. MEK1 displays 85% identity with MEK2. Although MEK1 and MEK2 have been found to be functionally similar, they may serve non-redundant functions. Both are required for cellular proliferation and may play a role in tumorigenesis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Surrounding threonine 286
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMEK1 Phospho (T286)
Antigen Species
Human
Gene ID5604
Gene Symbol
MAP2K1
Gene Name
mitogen-activated protein kinase kinase 1
Uniprot IDQ02750
Protein Name
Dual specificity mitogen-activated protein kinase kinase 1
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Phospho MEK1 immobilized on solid support.
The epitope recognized by A303-610A maps to a region of human MAPK/ERK kinase 1 (dual specificity mitogen-activiated protein kinase kinase 1) surrounding threonine 286 using the numbering given in entry NP_002746.1 (Gene ID 5604) when the threonine residue is phosphorylated.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A303-610A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-610A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-610AOpen
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Resources
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