Bethyl Laboratories

Rabbit anti-INT11 IHC Antibody Affinity Purified

Primary
Catalog #IHC-00309
TargetINT11
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
Detection of human INT11 by immunohistochemistry.
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Product Description

INT11 has been identified as a component of the twelve-subunit integrator complex that functions in RNA Pol II mediated 3’-end mRNA processing of the U1 and U2 small nuclear RNAs (snRNAs). INT11 is a beta-lactamase family member that has sequence similarity to the cleavage and polyadenylation specificity factors CPSF-73 and CPSF-100.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 550 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetINT11
Antigen Species
Human
Gene ID54973
Gene Symbol
INTS11
Gene Name
integrator complex subunit 11
Uniprot IDQ5TA45
Protein Name
Integrator complex subunit 11
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to INT11 immobilized on solid support.

The epitope recognized by IHC-00309 maps to a region between residue 550 and 600 of human integrator complex subunit 11 using the numbering given in entry NP_060341.2 (GeneID 54973).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.

Immunohistochemistry (IHC)1:100 - 1:500

Documents

Safety Data SheetPDF · IHC-00309Open
Product DatasheetPDF · IHC-00309 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00309 · Lot 1 (current lot)Open
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