Bethyl Laboratories
Rabbit anti-JARID1A/RBP2 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00479
TargetJARID1A/RBP2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
JARID1A/RBP2 is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. Some recently characterized members of the jmj family include JARID1A/RBP2, JARID1C, JMJD1A, JMJD1B, JMJD1C, JMJD2A, JMJD2C and JMJD2D. JARID1A/RBP2 functions to specifically demethylate lys-4 of histone 3 and interacts with the tumor suppressor protein pRb.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1640 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetJARID1A/RBP2
Antigen Species
Human
Gene ID5927
Gene Symbol
KDM5A
Gene Name
lysine demethylase 5A
Uniprot IDP29375
Protein Name
Lysine-specific demethylase 5A
Alternate Names
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to JARID1A/RBP2 immobilized on solid support.
The epitope recognized by IHC-00479 maps to a region between residue 1640 and 1690 of human Jumonji/ARID Domain-Containing Protein 1A/Retinoblastoma Binding Protein 2 Homolog 1 using the numbering given in entry NP_001036068.1 (GeneID 5927).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00479Open
Product DatasheetPDF · IHC-00479 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00479 · Lot 1 (current lot)Open
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