Rabbit anti-KLHL42 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Kelch-like protein 42 (KLHL42) is a substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL42) E3 ubiquitin ligase complex mediates the ubiquitination and subsequent degradation of KATNA1. Involved in microtubule dynamics throughout mitosis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9P2K6].
Alternate Names
Ctb9; cullin-3-binding protein 9; kelch domain containing 5; kelch domain-containing protein 5; kelch-like protein 42; KLHDC5
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.