Bethyl Laboratories
Rabbit anti-MEK1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00619
TargetMEK1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
MEK1 is an activator and kinase for MAP and ERK kinases. It is a serine/threonine kinase essential to the MAP kinase signal transduction pathway which is involved in a multitude of cellular processes such as proliferation, differentiation, gene activation, and development. MEK1 displays 85% identity with MEK2. Although MEK1 and MEK2 have been found to be functionally similar, they may serve non-redundant functions. Both are required for cellular proliferation and may play a role in tumorigenesis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 343 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetMEK1
Antigen Species
Human
Gene ID5604
Gene Symbol
MAP2K1
Gene Name
mitogen-activated protein kinase kinase 1
Uniprot IDQ02750
Protein Name
Dual specificity mitogen-activated protein kinase kinase 1
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MEK1 immobilized on solid support.
The epitope recognized by IHC-00619 maps to a region between residue 343 and 393 of human MAPK/ERK kinase 1 (Dual specificity mitogen-activated protein kinase kinase 1) using the numbering given in entry NP_002746.1 (GeneID 5604).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00619Open
Product DatasheetPDF · IHC-00619 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00619 · Lot 1 (current lot)Open
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