/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-MLH1 Recombinant Monoclonal Antibody [BLR112H]
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Primary Antibodies  

Rabbit anti-MLH1 Recombinant Monoclonal Antibody [BLR112H]

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

MLH1

Reactivity:

Human

Applications:

ICC

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR112H

Format:

Whole IgG

Immunogen:

between 450 and 500

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


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Additional Product Information

MLH1 is an integral component of the DNA mismatch repair (MMR) system that functions to recognize aberrant structures and signals for the repair of single base mismatches or loops in replicated DNA. MLH1 can heterodimerize with DNA mismatch repair protein MLH3 and with mismatch repair endonuclease PMS2. [taken from NCBI Entrez Gene (Gene ID: 4292)].

4292

MLH1

mutL homolog 1

P40692

DNA mismatch repair protein Mlh1

Alternate Names

COCA2; DNA mismatch repair protein Mlh1; FCC2; hMLH1; HNPCC; HNPCC2; mutL homolog 1, colon cancer, nonpolyposis type 2; MutL protein homolog 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.