Bethyl Laboratories
Rabbit anti-NARFL Antibody Affinity Purified
Primary
Catalog #A305-770A
TargetNARFL
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Small glutamine-rich tetratricopeptide repeat-containing protein beta (SGTB) is a co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96EQ0].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 225 and 275
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNARFL
Antigen Species
Human
Gene ID64428
Gene Symbol
CIAO3
Gene Name
nuclear prelamin A recognition factor like
Uniprot IDQ9H6Q4
Protein Name
Cytosolic Fe-S cluster assembly factor NARFL
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NARFL immobilized on solid support.
The epitope recognized by A305-770A maps to a region between residue 225 and 275 of human Cytosolic Fe-S cluster assembly factor NARFL using the numbering given in entry Q9H6Q4.1 (GeneID 64428).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A305-770A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-770A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-770AOpen
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Resources
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