Bethyl Laboratories
Rabbit anti-NHERF1 Antibody Affinity Purified
Primary
Catalog #A302-975A
TargetNHERF1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
NHERF1 (sodium-hydrogen exchanger regulatory factor 1) is a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer [taken from NCBI Entrez Gene (Gene ID: 9368)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 308 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetNHERF1
Antigen Species
Human
Gene ID9368
Gene Symbol
SLC9A3R1
Gene Name
SLC9A3 regulator 1
Uniprot IDO14745
Protein Name
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
Alternate Names
EBP50 • ezrin-radixin-moesin binding phosphoprotein-50 • Ezrin-radixin-moesin-binding phosphoprotein 50 • Na(+)/H(+) exchange regulatory cofactor NHE-RF1 • Na+/H+ exchange regulatory co-factor • NHERF • NHERF1 • NHERF-1 • NPHLOP2 • regulatory cofactor of Na(+)/H(+) exchanger • Sodium-hydrogen exchanger regulatory factor 1 • solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 • solute carrier family 9 isoform A3 regulatory factor 1 • solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NHERF1 immobilized on solid support.
The epitope recognized by A302-975A maps to a region between residue 325 and 358 of human Sodium/Hydrogen Exchanger Regulatory Factor 1 using the numbering given in entry NP_004243.1 (GeneID 9368).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-975A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-975A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-975AOpen
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