Bethyl Laboratories
Rabbit anti-NUP214 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00103
TargetNUP214
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF, IHC
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Product Description
Nucleoporins are the main components of the nuclear pore complex that provides a conduit across the nuclear envelope for the transport of macromolecules between the nucleus and cytoplasm. Nucleoporin 214 (NUP214) is required for CRM1-mediated nuclear protein export as well as cell cycle progression.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1250 and 1300
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
500 µg/ml
Target Identity
TargetNUP214
Antigen Species
Human
Gene ID8021
Gene Symbol
NUP214
Gene Name
nucleoporin 214
Uniprot IDP35658
Protein Name
Nuclear pore complex protein Nup214
Alternate Names
Product Information
Applications
ICC-IF, IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NUP214 immobilized on solid support.
The epitope recognized by IHC-00103 maps to a region between residue 1250 and 1300 of human Nuceloporin 214 using the numbering given in entry NP_005076.3 (GeneID 8021).
Applications
Formalin-Fixed Paraffin-Embedded Tissue 1:100 - 1:500:
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Formaldehyde-Fixed Cells 1:100 - 1:500.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:100 - 1:500
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00103Open
Product DatasheetPDF · IHC-00103 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00103 · Lot 1 (current lot)Open
Citations / Publications
Services
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