Bethyl Laboratories
Rabbit anti-Occludin Recombinant Monoclonal Antibody [BLR126H]
Monoclonal Antibodies
Catalog #A700-126
TargetOccludin
HostRabbit
CloneBLR126H
ReactivityHuman
ApplicationIP, WB
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Product Description
Occludin may play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q16625].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR126H
Host
Rabbit
Immunogen
Between 472 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
100 µg/ml
Target Identity
TargetOccludin
Antigen Species
Human
Gene ID100506658
Gene Symbol
OCLN
Gene Name
occludin
Uniprot IDQ16625
Protein Name
Occludin
Alternate Names
Product Information
Applications
IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 472 and the C-terminus (residue 522) of human occludin using the numbering given in entry NP_001192183.1 (Gene ID 100506658).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-126 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-126 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-126 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-126Open
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Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
Explore Simple Western™
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