/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-BRD4 Recombinant Monoclonal Antibody [BL-149-2H5]
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Primary Antibodies  

Rabbit anti-BRD4 Recombinant Monoclonal Antibody [BL-149-2H5]

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (17)
Target:

BRD4

Reactivity:

Human

,

Mouse

Applications:

ChIP

,

Flow Cyt

,

ICC

,

ICC-IF

,

IHC

,

IP

,

WB

Platforms:

COMET™

,

PhenoImager™ HT

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BL-149-2H5

Format:

Whole IgG

Immunogen:

Between 1312 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (10 blots)

20 µl (2 blots)

100 µg (BSA-Free)

$127.00 $794.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
100 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change
Production Procedures

Recombinant antibody was purified from cell culture supernatant.

The epitope recognized by A700-004-T maps to a region between residue 1312 and 1362 of human bromodomain-containing protein 4 using the numbering given in entry NP_490597.1 (GeneID 23476).

Recombinant antibody was purified from cell culture supernatant.

The epitope recognized by A700-004 maps to a region between residue 1312 and 1362 of human bromodomain-containing protein 4 using the numbering given in entry NP_490597.1 (GeneID 23476).

Country of Origin

USA

Additional Product Information

Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated chromatin. It was originally identified as MCAP (mitotic chromosome associated protein) due to its association with mitotic chromosomes. BRD4 also associates with several transcription complexes such as the Mediator complexes and P-TEFb complexes and is considered to function as a transcriptional co-factor.

23476

BRD4

bromodomain containing 4

O60885

Bromodomain-containing protein 4

Alternate Names

bromodomain-containing protein 4; CAP; chromosome-associated protein; HUNK1; HUNKI; MCAP; Protein HUNK1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.