Bethyl Laboratories
Rabbit anti-RPSA Antibody Affinity Purified
Primary
Catalog #A305-299A
TargetRPSA
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
RPSA is required for the assembly and/or stability of the 40S ribosomal subunit. RPSA is required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. RPSA also functions as a cell surface receptor for laminin and plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. RPSA may play a role in cell fate determination and tissue morphogenesis. It acts as a PPP1R16B-dependent substrate of PPP1CA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P08865].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 245 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRPSA
Antigen Species
Human
Gene ID3921
Gene Symbol
RPSA
Gene Name
ribosomal protein SA
Uniprot IDP08865
Protein Name
40S ribosomal protein SA
Alternate Names
37 kDa laminin receptor • 37 kDa laminin receptor precursor • 37/67 kDa laminin receptor • 37LRP • 40S ribosomal protein SA • 67 kDa laminin receptor • 67LR • colon carcinoma laminin-binding protein • ICAS • LAMBR • Laminin receptor 1 • laminin receptor 1 (67kD, ribosomal protein SA) • laminin-binding protein precursor p40 • lamR • LAMR1 • LBP • LBP/p40 • LRP • LRP/LR • multidrug resistance-associated protein MGr1-Ag • NEM/1CHD4 • p40 • SA • small ribosomal subunit protein uS2
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RPSA immobilized on solid support.
The epitope recognized by A305-299A maps to a region between residue 245 to 295 of human 40S ribosomal protein SA using the numbering given in entry P08865.4 (GeneID 3921).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A305-299A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-299A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-299AOpen
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