Bethyl Laboratories
Rabbit anti-Pericentrin/Kendrin Antibody Affinity Purified
Primary
Catalog #A301-348A
TargetPericentrin/Kendrin
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF, IP, WB
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Product Description
Pericentrin (PCNT) is a large centrosomal protein that binds calmodulin and mediates assembly of the mitotic spindle apparatus. Depletion of PCNT has been shown to cause apoptosis and activation of the G2/M checkpoint. Recently, mutations in the pericentrin gene have been identified as the cause of primordial dwarfism type II (MOPDII) and Seckel syndrome. Additionally, in cells of Seckel syndrome patients, PCNT has been found to be important to the DNA damage response and ATR-dependent checkpoint signaling.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 175 and 225
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPericentrin/Kendrin
Antigen Species
Human
Gene ID5116
Gene Symbol
PCNT
Gene Name
pericentrin
Uniprot IDO95613
Protein Name
Pericentrin
Alternate Names
KEN • kendrin • MOPD2 • PCN • PCNT2 • PCNTB • PCTN2 • pericentrin • pericentrin-2 • pericentrin-380 • pericentrin-B • SCKL4
Product Information
Applications
ICC-IF, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Pericentrin/Kendrin immobilized on solid support.
The epitope recognized by A301-348A maps to a region between residue 175 and 225 of human Pericentrin/Kendrin using the numbering given in entry NP_006022.3 (GeneID 5116).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:1000 - 1:5000. Formaldehyde or Acetone fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A301-348A · Lot 4 (current lot)Open
Product DatasheetPDF · A301-348A · Lot 4 (current lot)Open
Safety Data SheetPDF · A301-348AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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