Bethyl Laboratories
Rabbit anti-Pericentrin/Kendrin IHC Antibody Affinity Purified
Primary
Catalog #IHC-00264
TargetPericentrin/Kendrin
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF
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Product Description
Pericentrin (PCNT) is a large centrosomal protein that binds calmodulin and mediates assembly of the mitotic spindle apparatus. Depletion of PCNT has been shown to cause apoptosis and activation of the G2/M checkpoint. Recently, mutations in the pericentrin gene have been identified as the cause of primordial dwarfism type II (MOPDII) and Seckel syndrome. Additionally, in cells of Seckel syndrome patients, PCNT has been found to be important to the DNA damage response and ATR-dependent checkpoint signaling.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 175 and 225
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetPericentrin/Kendrin
Antigen Species
Human
Gene ID5116
Gene Symbol
PCNT
Gene Name
pericentrin
Uniprot IDO95613
Protein Name
Pericentrin
Alternate Names
KEN • kendrin • MOPD2 • PCN • PCNT2 • PCNTB • PCTN2 • pericentrin • pericentrin-2 • pericentrin-380 • pericentrin-B • SCKL4
Product Information
Applications
ICC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Pericentrin/Kendrin immobilized on solid support.
The epitope recognized by IHC-00264 maps to a region between residue 175 and 225 of human Pericentrin/Kendrin using the numbering given in entry NP_006022.3 (GeneID 5116).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Formaldehyde-Fixed Cells 1:100 - 1:500:
Permeabilization with Triton-X 100 is recommended.
Acetone-Fixed Cells: 1:100 - 1:500.
Immunocytochemistry-Immunofluoresence (ICC-IF)Formaldehyde-Fixed Cells 1:100 - 1:500: Permeabilization with Triton-X 100 is recommended
Documents
Safety Data SheetPDF · IHC-00264Open
Product DatasheetPDF · IHC-00264 · Lot 2 (current lot)Open
Product DatasheetPDF · IHC-00264 · Lot 2 (current lot)Open
Citations / Publications
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Resources
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