Bethyl Laboratories
Rabbit anti-VAT1 Antibody Affinity Purified
Primary
Catalog #A305-561A
TargetVAT1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
Vesicle amine transport 1 (VAT1) is an abundant integral membrane protein of cholinergic synaptic vesicles and is thought to be involved in vesicular transport. It belongs to the quinone oxidoreductase subfamily of zinc-containing alcohol dehydrogenase proteins [taken from NCBI Entrez Gene (Gene ID: 10493)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetVAT1
Antigen Species
Human
Gene ID10493
Gene Symbol
VAT1
Gene Name
vesicle amine transport 1
Uniprot IDQ99536
Protein Name
Synaptic vesicle membrane protein VAT-1 homolog
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to VAT1 immobilized on solid support.
The epitope recognized by A305-561A maps to a region between residue 1 to 50 of human Synaptic vesicle membrane protein VAT-1 homolog using the numbering given in entry Q99536.2 (GeneID 10493).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A305-561A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-561A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-561AOpen
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