Bethyl Laboratories
Rabbit anti-Phospho KAP-1 (S824) Antibody Affinity Purified
Primary
Catalog #A300-767A
TargetKAP-1 Phospho (S824)
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationICC, ICC-IF, IHC, IP, WB
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Product Description
KAP-1 (KRAB-Associated Protein 1) is a member of the tripartite motif RBCC/Trim domain family that contains a RING finger domain, B boxes, and an alpha helical coiled coil region. KAP-1 functions as a corepressor by interacting with the KRAB domain of KRAB zinc-finger transcriptional repressors. KAP-1 also appears to function as a scaffold for chromatin-remodeling complexes involved in transcriptional repression.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Surrounding serine 824
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetKAP-1 Phospho (S824)
Antigen Species
Human
Gene ID10155
Gene Symbol
TRIM28
Gene Name
tripartite motif containing 28
Uniprot IDQ13263
Protein Name
Transcription intermediary factor 1-beta
Alternate Names
E3 SUMO-protein ligase TRIM28 • KAP1 • KAP-1 • KRAB [Kruppel-associated box domain]-associated protein 1 • KRAB-associated protein 1 • KRAB-interacting protein 1 • KRIP-1 • nuclear corepressor KAP-1 • PPP1R157 • protein phosphatase 1, regulatory subunit 157 • RING finger protein 96 • RING-type E3 ubiquitin transferase TIF1-beta • RNF96 • TF1B • TIF1B • TIF1-beta • transcription intermediary factor 1-beta • transcriptional intermediary factor 1-beta • Tripartite motif-containing protein 28
Product Information
Applications
ICC, ICC-IF, IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Phospho KAP-1 (S824) immobilized on solid support.
Immunogen was a synthetic phosphorylated peptide, which represented a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry (ICC)1:200 - 1:1000
Immunocytochemistry-Immunofluoresence (ICC-IF)1:200 - 1:1000
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-767A · Lot 6 (current lot)Open
Product DatasheetPDF · A300-767A · Lot 6 (current lot)Open
Safety Data SheetPDF · A300-767AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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