Bethyl Laboratories
Rabbit anti-STAT6 Recombinant Monoclonal Antibody [BLR147J]
Monoclonal Antibodies
Catalog #A700-147
TargetSTAT6
HostRabbit
CloneBLR147J
ReactivityMouse, Human
ApplicationICC, IHC, IP, WB
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Product Description
STAT6 is part of the STAT (signal transducers and activators of transcription) family of transcription factors that relays signals from cytokine receptors to the nucleus where they act as transcriptional activators. STAT6 mediates signals that originate from the IL-4 receptor via JAKs (Janus Kinases) and activates a gene program involved in B- and T- cell differentiation, proliferation, and apoptosis.
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR147J
Host
Rabbit
Immunogen
Between 600 and 650
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
25 µg/ml
Target Identity
TargetSTAT6
Antigen Species
Human
Gene ID6778
Gene Symbol
STAT6
Gene Name
signal transducer and activator of transcription 6
Uniprot IDP42226
Protein Name
Signal transducer and activator of transcription 6
Product Information
Applications
ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 600 and 650 of human Signal Transducer and Activator of Transcription 6 (STAT6) using the numbering given in entry NP_001171549.1 (Gene ID 6778).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-147 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-147 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-147 · Lot 201012Open
Safety Data SheetPDF · A700-147Open
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
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