/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Phospho KAP-1 (S824) Recombinant Monoclonal Antibody [BL-246-7B5]
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Primary Antibodies  

Rabbit anti-Phospho KAP-1 (S824) Recombinant Monoclonal Antibody [BL-246-7B5]

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (5)
Target:

KAP-1 Phospho (S824)

Reactivity:

Human

,

Mouse

Applications:

Flow Cyt

,

ICC-IF

,

IHC

,

IP

,

WB

Platforms:

Simple Western

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BL-246-7B5

Format:

Whole IgG

Immunogen:

Surrounding serine 824

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (10 blots)

20 µl (2 blots)

100 µg (BSA-Free)

$127.00 $794.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
100 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change
Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a synthetic phosphorylated peptide, which represented a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155).

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a synthetic phosphorylated peptide, which represented a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155).

Country of Origin

USA

Additional Product Information

KAP-1 (KRAB-Associated Protein 1) is a member of the tripartite motif RBCC/Trim domain family that contains a RING finger domain, B boxes, and an alpha helical coiled coil region. KAP-1 functions as a corepressor by interacting with the KRAB domain of KRAB zinc-finger transcriptional repressors. KAP-1 also appears to function as a scaffold for chromatin-remodeling complexes involved in transcriptional repression.

10155

TRIM28

tripartite motif containing 28

Q13263

Transcription intermediary factor 1-beta

Alternate Names

E3 SUMO-protein ligase TRIM28; KAP1; KAP-1; KRAB Kruppel-associated box domain-associated protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP-1; nuclear corepressor KAP-1; PPP1R157; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; RNF96; TF1B; TIF1B; TIF1-beta; transcription intermediary factor 1-beta; transcriptional intermediary factor 1-beta; Tripartite motif-containing protein 28

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.