Bethyl Laboratories
Rabbit anti-Phospho KAP-1 (S824) Recombinant Monoclonal Antibody [BL-246-7B5]
Monoclonal Antibodies
Catalog #A700-013
TargetKAP-1 Phospho (S824)
HostRabbit
CloneBL-246-7B5
ReactivityMouse, Human
ApplicationFlow Cyt, ICC-IF, IHC, IP, WB
Image 1 of 8
local_shipping
Order within 4 hours and 56 minutes for shipment by Tuesday, November 17
Product Description
KAP-1 (KRAB-Associated Protein 1) is a member of the tripartite motif RBCC/Trim domain family that contains a RING finger domain, B boxes, and an alpha helical coiled coil region. KAP-1 functions as a corepressor by interacting with the KRAB domain of KRAB zinc-finger transcriptional repressors. KAP-1 also appears to function as a scaffold for chromatin-remodeling complexes involved in transcriptional repression.
Specifications
Clonality
Recombinant Monoclonal
Clone
BL-246-7B5
Host
Rabbit
Immunogen
Surrounding serine 824
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
100 µg/ml
Target Identity
TargetKAP-1 Phospho (S824)
Antigen Species
Human
Gene ID10155
Gene Symbol
TRIM28
Gene Name
tripartite motif containing 28
Uniprot IDQ13263
Protein Name
Transcription intermediary factor 1-beta
Alternate Names
E3 SUMO-protein ligase TRIM28 • KAP1 • KAP-1 • KRAB [Kruppel-associated box domain]-associated protein 1 • KRAB-associated protein 1 • KRAB-interacting protein 1 • KRIP-1 • nuclear corepressor KAP-1 • PPP1R157 • protein phosphatase 1, regulatory subunit 157 • RING finger protein 96 • RING-type E3 ubiquitin transferase TIF1-beta • RNF96 • TF1B • TIF1B • TIF1-beta • transcription intermediary factor 1-beta • transcriptional intermediary factor 1-beta • Tripartite motif-containing protein 28
Product Information
Applications
Flow Cyt, ICC-IF, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a synthetic phosphorylated peptide, which represented a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:100 - 1:500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
SW-Size1:10 - 1:250
Western Blot (WB)1:1000
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Documents
Product DatasheetPDF · A700-013 · Lot 4 (current lot)Open
Product DatasheetPDF · A700-013 · Lot 4 (current lot)Open
Product DatasheetPDF · A700-013 · Lot 211217Open
Safety Data SheetPDF · A700-013Open
Citations / Publications
Related Products
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request Customization
Custom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal Development
VHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH Discovery
Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
Explore Simple Western™
1