Bethyl Laboratories
Rabbit anti-Phospho KAP-1 (S824) IHC Antibody Affinity Purified
Primary
Catalog #IHC-00073
TargetKAP-1 Phospho (S824)
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF
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Product Description
KAP-1 (KRAB-Associated Protein 1) is a member of the tripartite motif RBCC/Trim domain family that contains a RING finger domain, B boxes, and an alpha helical coiled coil region. KAP-1 functions as a corepressor by interacting with the KRAB domain of KRAB zinc-finger transcriptional repressors. KAP-1 also appears to function as a scaffold for chromatin-remodeling complexes involved in transcriptional repression.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Surrounding serine 824
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
50 µg/ml
Target Identity
TargetKAP-1 Phospho (S824)
Antigen Species
Human
Gene ID10155
Gene Symbol
TRIM28
Gene Name
tripartite motif containing 28
Uniprot IDQ13263
Protein Name
Transcription intermediary factor 1-beta
Alternate Names
E3 SUMO-protein ligase TRIM28 • KAP1 • KAP-1 • KRAB Kruppel-associated box domain-associated protein 1 • KRAB-associated protein 1 • KRAB-interacting protein 1 • KRIP-1 • nuclear corepressor KAP-1 • PPP1R157 • protein phosphatase 1, regulatory subunit 157 • RING finger protein 96 • RING-type E3 ubiquitin transferase TIF1-beta • RNF96 • TF1B • TIF1B • TIF1-beta • transcription intermediary factor 1-beta • transcriptional intermediary factor 1-beta • Tripartite motif-containing protein 28
Product Information
Applications
ICC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Phospho KAP-1 (S824) immobilized on solid support.
Immunogen was a synthetic phosphorylated peptide, which represented a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155).
Applications
Formaldehyde-fixed cells 1:100 - 1:500:
Permeabilization with Triton-X 100 is recommended.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00073Open
Product DatasheetPDF · IHC-00073 · Lot 2 (current lot)Open
Product DatasheetPDF · IHC-00073 · Lot 2 (current lot)Open
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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Simple Western™ Resources
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