Bethyl Laboratories
Rabbit anti-Phospho RelA (S529) Antibody Affinity Purified
Primary
Catalog #A302-063A
TargetRelA Phospho (S529)
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
RelA is one of five monomeric subunits that are part of the Rel family of proteins that dimerize to form the NF-kappa B (nuclear factor that binds the kappa immunoglobulin light chain gene enhancer) transcription factor. Members of the Rel family of proteins include NF-kappa B1/p50, NF-kappa B2/p52, RelA/p65, c-Rel, and RelB. Each Rel member bears a characteristic 300 amino acid N-terminal Rel homology domain (RHD) that functions to mediate DNA binding, dimerization, nuclear localization, and inhibitor association.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Surrounding serine 529
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRelA Phospho (S529)
Antigen Species
Human
Gene ID5970
Gene Symbol
RELA
Gene Name
RELA proto-oncogene, NF-kB subunit
Uniprot IDQ04206
Protein Name
Transcription factor p65
Alternate Names
NF-kappa-B p65delta3 • NF-kappa-B transcription factor p65 • NFKB3 • nuclear factor NF-kappa-B p65 subunit • nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 • p65 • transcription factor p65 • v-rel avian reticuloendotheliosis viral oncogene homolog A • v-rel reticuloendotheliosis viral oncogene homolog A
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Phospho RelA (S529) immobilized on solid support.
The epitope recognized by A302-063A maps to a region of human v-rel reticuloendotheliosis viral oncogene homolog A surrounding serine 529 when serine 529 is phosphorylated using the numbering given in entry NP_068810.2 (GeneID 5970).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-063A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-063A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-063AOpen
Services
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