Bethyl Laboratories
Rabbit anti-PRPF4B Antibody Affinity Purified
Primary
Catalog #A301-665A
TargetPRPF4B
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, WB
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Product Description
PRPF4B (pre-mRNA processing factor 4 homolog B) is a cyclin dependent kinase-like kinase (Clk) identified in splicesome complexes and implicated to play a role in pre-mRNA splicing.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetPRPF4B
Antigen Species
Human
Gene ID8899
Gene Symbol
PRPF4B
Gene Name
pre-mRNA processing factor 4B
Uniprot IDQ13523
Protein Name
Serine/threonine-protein kinase PRP4 homolog
Product Information
Applications
IHC, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PRPF4B immobilized on solid support.
The epitope recognized by A301-665A maps to a region between residue 1 and 50 of human PRP4 pre-mRNA processing factor 4 homolog B using the numbering given in entry NP_003904.3 (GeneID 8899).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-665A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-665A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-665AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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