Bethyl Laboratories
Rabbit anti-RPL7 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00455
TargetRPL7
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IHC-IF
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Product Description
RPL7 is a component of the 60S ribosomal subunit. Ribosomes are composed of a small 40S subunit and a large 60S subunit that function to catalyze protein synthesis. RPL7 is a member of the L30P family of ribosomal proteins and can bind both DNA and RNA in vitro. RPL7 is a cytoplasmic protein implicated to play a regulatory role in translation. It has also been identified as an auto-antigen in patients with autoimmune diseases such as lupus erythematosus.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 198 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetRPL7
Antigen Species
Human
Gene ID6129
Gene Symbol
RPL7
Gene Name
ribosomal protein L7
Uniprot IDP18124
Protein Name
60S ribosomal protein L7
Alternate Names
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RPL7 immobilized on solid support.
The epitope recognized by IHC-00455 maps to a region between residue 200 and the C-terminus (residue 248) of human Ribosomal Protein L7 using the numbering given in entry NP_000962.2 (GeneID 6129).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00455Open
Product DatasheetPDF · IHC-00455 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00455 · Lot 1 (current lot)Open
Citations / Publications
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