/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-SIRP alpha Recombinant Monoclonal Antibody [BLR049F]
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Primary Antibodies  

Rabbit anti-SIRP alpha Recombinant Monoclonal Antibody [BLR049F]

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

SIRP alpha

Reactivity:

Human

Applications:

ICC

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR049F

Format:

Whole IgG

Immunogen:

residues 31-368 (ECD)

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
250 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change

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Additional Product Information

SIRP alpha is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRP alpha is a cell surface receptor for CD47 and CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. [taken from NCBI Entrez Gene (Gene ID: 140885)].

140885

SIRPA

signal regulatory protein alpha

P78324

Tyrosine-protein phosphatase non-receptor type substrate 1

Alternate Names

BIT; Brain Ig-like molecule with tyrosine-based activation motifs; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; CD antigen CD172a; CD172 antigen-like family member A; CD172A; inhibitory receptor SHPS-1; macrophage fusion receptor; MFR; MYD-1; myd-1 antigen; P84; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; SIRP; Sirp-alpha-1; Sirp-alpha-2; Sirp-alpha-3; tyrosine phosphatase SHP substrate 1; tyrosine-protein phosphatase non-receptor type substrate 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.