Bethyl Laboratories
Rabbit anti-T-bet/TBX21 Recombinant Monoclonal Antibody [BLR110H]
Monoclonal Antibodies
Catalog #A700-110
TargetT-bet/TBX21
HostRabbit
CloneBLR110H
ReactivityHuman
ApplicationFlow Cyt, ICC, IHC, IP, WB
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Product Description
T-bet/TBX21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. TBX21 is a lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and activating transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. [taken from NCBI Entrez Gene (Gene ID: 30009)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR110H
Host
Rabbit
Immunogen
Between 485 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetT-bet/TBX21
Antigen Species
Human
Gene ID30009
Gene Symbol
TBX21
Gene Name
T-box 21
Uniprot IDQ9UL17
Protein Name
T-box transcription factor TBX21
Product Information
Applications
Flow Cyt, ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 485 and the C-terminus (residue 535) of human T-box transcription factor TBX21 using the numbering given in entry NP_037483.1 (Gene ID 30009).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed and permeabilized cells were stained with 0.05 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-110 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-110 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-110Open
Citations / Publications
Services
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