Bethyl Laboratories

Rabbit anti-UBXN4 Antibody Affinity Purified

Primary
Catalog #A305-635A
TargetUBXN4
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
Detection of human UBXN4 by western blot.
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Product Description

UBX domain-containing protein 4 (UBXN4) is Involved in endoplasmic reticulum-associated protein degradation (ERAD). It acts as a platform to recruit both UBQLN1 and VCP to the ER during ERAD (PubMed:19822669) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q92575].

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 458 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetUBXN4
Antigen Species
Human
Gene ID23190
Gene Symbol
UBXN4
Gene Name
UBX domain protein 4
Uniprot IDQ92575
Protein Name
UBX domain-containing protein 4
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to UBXN4 immobilized on solid support.

The epitope recognized by A305-635A maps to a region between residue 458 to 508 of human UBX domain-containing protein 4 using the numbering given in entry Q92575.2 (GeneID 23190).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western Blot (WB)1:2000 - 1:10,000

Documents

Product DatasheetPDF · A305-635A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-635A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-635AOpen
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