Bethyl Laboratories

Rabbit anti-USP47 IHC Antibody Affinity Purified

Primary
Catalog #IHC-00235
TargetUSP47
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IHC-IF
Detection of human USP47 by immunohistochemistry.
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Product Description

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific peptidase (USP47) is a UBP.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1325 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetUSP47
Antigen Species
Human
Gene ID55031
Gene Symbol
USP47
Gene Name
ubiquitin specific peptidase 47
Uniprot IDQ96K76
Protein Name
Ubiquitin carboxyl-terminal hydrolase 47
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to USP47 immobilized on solid support.

The epitope recognized by IHC-00235 maps to a region between residue 1237 and the C-terminus (residue 1287) of human ubiquitin specific peptidase 47 using the numbering given in entry NP_060414.3 (GeneID 55031).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.

Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500

Documents

Safety Data SheetPDF · IHC-00235Open
Product DatasheetPDF · IHC-00235 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00235 · Lot 1 (current lot)Open

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