Bethyl Laboratories
Rabbit anti-VISTA Recombinant Monoclonal Antibody [BLR035F]
Monoclonal Antibodies
Catalog #A700-035
TargetVISTA
HostRabbit
CloneBLR035F
ReactivityHuman
ApplicationFlow Cyt, ICC, IHC, IHC-IF, IP, WB
Image 1 of 6
local_shipping
Order within 10 hours and 49 minutes for shipment by Tuesday, November 10
Product Description
VISTA is an immunoregulatory receptor which inhibits the T-cell response (PubMed:24691993). It may promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity). VISTA may stimulate MMP14-mediated MMP2 activation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H7M9].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR035F
Host
Rabbit
Immunogen
Between 261 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
100 µg/ml
Target Identity
TargetVISTA
Antigen Species
Human
Gene ID64115
Gene Symbol
VSIR
Gene Name
V-set immunoregulatory receptor
Uniprot IDQ9H7M9
Protein Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
Alternate Names
B7H5 • B7-H5 • C10orf54 • DD1alpha • Death Domain1alpha • GI24 • PD-1H • PDCD1 homolog • platelet receptor GI24 • PP2135 • SISP1 • sisp-1 • stress-induced secreted protein-1 • V-domain Ig suppressor of T cell activation • VISTA • V-set domain-containing immunoregulatory receptor • V-set immunoregulatory receptor • V-type immunoglobulin domain-containing suppressor of T-cell activation
Product Information
Applications
Flow Cyt, ICC, IHC, IHC-IF, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 250 and the C-terminus (residue 311) of human V-type immunoglobulin domain-containing suppressor of T-cell activation precursor using the numbering given in entry NP_071436.1 (Gene ID 64115).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 2 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-035 · Lot 3 (current lot)Open
Product DatasheetPDF · A700-035 · Lot 3 (current lot)Open
Product DatasheetPDF · A700-035 · Lot 240813Open
Safety Data SheetPDF · A700-035Open
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1