Bethyl Laboratories
Rabbit anti-WDFY3 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00372
TargetWDFY3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
WDFY3 (WD repeat and FYVE domain –containing protein 3) was identified in a screen for phosphatidylinositol -3-phosphate [PtdIns(3)P]- binding proteins. The FYVE domain is a zinc binding domain found in many PtdIns(3)-binding proteins and is proposed to target proteins to membrane lipids. WDFY3 has been shown localized to the nuclear membrane and within autophagic membranes and it is proposed to function as a link between protein aggregates and the autophagic machinery.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 500 and 550
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetWDFY3
Antigen Species
Human
Gene ID23001
Gene Symbol
WDFY3
Gene Name
WD repeat and FYVE domain containing 3
Uniprot IDQ8IZQ1
Protein Name
WD repeat and FYVE domain-containing protein 3
Alternate Names
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WDFY3 immobilized on solid support.
The epitope recognized by IHC-00372 maps to a region between residue 500 and 550 of human WD repeat and FYVE domain containing 3 using the numbering given in entry NP_055806.2 (GeneID 23001).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00372Open
Product DatasheetPDF · IHC-00372 · Lot 2 (current lot)Open
Product DatasheetPDF · IHC-00372 · Lot 2 (current lot)Open
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Resources
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