Sheep anti-Bovine Albumin Antibody Biotinylated
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.05% Pro-Clean 400
Request Formulation Change Serum containing 0.09% Sodium Azide
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Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine albumin. This antibody may cross react with albumin from other species. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine albumin. This antibody may cross react with albumin from other species. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine albumin. This antibody may cross react with albumin from other species. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine albumin. This antibody may cross react with albumin from other species. By immunoelectrophoresis, the antiserum reacts specifically with bovine albumin. No antibody was detected against other bovine serum proteins.
This antibody may cross react with albumin from other species.
Additional Product Information
Albumin is a serum protein responsible for pH maintenance and the transport of a variety of compounds, such as fatty acids, amino acids, and hormones. It is also capable of binding to water molecules and modulates oncotic pressure.
Applications
Not all listed applications have been specifically tested by our laboratory.
For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP) or double diffusion Ouchterlony (DD)).
Optimal working dilutions should be determined experimentally by the investigator.