Bethyl Laboratories
Sheep anti-Bovine Albumin Cross-Adsorbed Antibody Affinity Purified
Primary
Catalog #A10-213A
TargetBovine Albumin
HostSheep
ClonalityPolyclonal
ReactivityBovine
ApplicationELISA, ICC, IHC, WB
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Product Description
Albumin is a serum protein responsible for pH maintenance and the transport of a variety of compounds, such as fatty acids, amino acids, and hormones. It is also capable of binding to water molecules and modulates oncotic pressure.
Specifications
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Reactivity
Bovine
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
0.5 mg/ml
Target Identity
TargetBovine Albumin
Antigen Species
Bovine
Gene ID280717
Gene Symbol
ALB
Gene Name
Bovine albumin
Uniprot IDP02769
Protein Name
Serum albumin
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
Antiserum was cross adsorbed using human, mouse and pig immunosorbents to remove cross reactive Antibodies. The antibody to bovine albumin was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with bovine albumin. Less than 1% cross reactivity to human, mouse and pig albumin was detected. This antibody may cross react with albumin from other species.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:20,000; for coating plates 1:50 - 1:250
Immunocytochemistry (ICC)1:100 - 1:1000
Immunohistochemistry (IHC)1:100 - 1:1000
Western Blot (WB)1:1000 - 1:20,000
Documents
Safety Data SheetPDF · A10-213AOpen
Product DatasheetPDF · A10-213A · Lot 240718 (current lot)Open
Safety Data SheetPDF · A10-213AOpen
Product DatasheetPDF · A10-213A · Lot 17Open
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