Bethyl Laboratories
Mouse anti-CD74 Monoclonal Antibody Purified [LN2]
Monoclonal Antibodies
Catalog #A500-023A
TargetCD74
HostMouse
CloneLN2
ReactivityHuman
ApplicationFlow Cyt, ICC, IHC, WB
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Product Description
CD74 plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. CD74 also serves as cell surface receptor for the cytokine MIF. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P04233].
Specifications
Clonality
Monoclonal
Clone
LN2
Host
Mouse
Immunogen
Human SU-DHL-4 lymphoma cells
Isotype
IgG1
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetCD74
Antigen Species
Human
Gene ID972
Gene Symbol
CD74
Gene Name
CD74 molecule
Uniprot IDP04233
Protein Name
HLA class II histocompatibility antigen gamma chain
Alternate Names
CD antigen CD74 • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) • CD74 molecule, major histocompatibility complex, class II invariant chain • DHLAG • gamma chain of class II antigens • HLA class II histocompatibility antigen gamma chain • HLADG • HLA-DR antigens-associated invariant chain • HLA-DR-gamma • Ia antigen-associated invariant chain • Ia-associated invariant chain • Ia-GAMMA • II • MHC HLA-DR gamma chain • p33
Product Information
Applications
Flow Cyt, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Monoclonal antibody was purified from cell culture supernatant.
Immunogen was human SU-DHL-4 lymphoma cells.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Goat anti-Mouse IgG Heavy and Light Chain Antibody (Cat. No. A90-216P).
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A500-023A · Lot 1Open
Product DatasheetPDF · A500-023A · Lot 2 (current lot)Open
Product DatasheetPDF · A500-023A · Lot 1Open
Product DatasheetPDF · A500-023A · Lot 2 (current lot)Open
Safety Data SheetPDF · A500-023AOpen
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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