Bethyl Laboratories
Sheep anti-Mouse IgG Heavy and Light Chain Antibody Affinity Purified
Secondary
Catalog #A90-146A
TargetMouse IgG Heavy and Light Chain
HostSheep
ClonalityPolyclonal
ReactivityMouse
ApplicationELISA, ICC, IHC, WB
Image 1 of 1
local_shipping
Order within 22 hours and 22 minutes for shipment by today
Product Description
Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.
Specifications
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme: antibody protein ratio is 1:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2,000
Immunohistochemistry (IHC)1:200 - 1:2,000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A90-146AOpen
Product DatasheetPDF · A90-146A · Lot 1 (current lot)Open
Safety Data SheetPDF · A90-146AOpen
Product DatasheetPDF · A90-146A · Lot 4Open
Safety Data SheetPDF · A90-146AOpen
Product DatasheetPDF · A90-146A · Lot 8Open
Safety Data SheetPDF · A90-146AOpen
Product DatasheetPDF · A90-146A · Lot 14Open
Related Products
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request Customization
Custom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal Development
VHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH Discovery
Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
Explore Simple Western™
1