Bethyl Laboratories
Rabbit anti-PARG Antibody Affinity Purified
Primary
Catalog #A305-410A
TargetPARG
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Image 1 of 1
local_shipping
Order within 3 hours and 56 minutes for shipment by Tuesday, November 10
Product Description
Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. PARG is found in many tissues and may be subject to proteolysis generating smaller, active products [taken from NCBI Entrez Gene (Gene ID: 8505)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 200 and 250
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPARG
Antigen Species
Human
Gene ID8505
Gene Symbol
PARG
Gene Name
poly(ADP-ribose) glycohydrolase
Uniprot IDQ86W56
Protein Name
Poly(ADP-ribose) glycohydrolase
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PARG immobilized on solid support.
The epitope recognized by A305-410A maps to a region between residue 200 to 250 of human Poly using the numbering given in entry Q86W56.1 (GeneID 8505).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A305-410A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-410A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-410AOpen
Related Products
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request Customization
Custom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal Development
VHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH Discovery
Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
Explore Simple Western™
1