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Rabbit anti-Mouse IgG Heavy and Light Chain Cross-Adsorbed Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Target:
Mouse IgG Heavy and Light Chain
Reactivity:
Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
HRP, Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.5 mg)

HRP (0.5 mg)

$84.00 $123.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse
Antigen Species
Mouse
Minimal Reactivity
Bovine, Chicken, Goat, Horse, Human, Pig
Concentration
0.5 mg/ml
Storage
2 - 8 °C
Shelf Life
2 years from date of receipt, 1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change
Production Procedures
Antiserum was cross adsorbed using bovine, chicken, goat, horse, human and pig immunosorbents to remove cross reactive Antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat. Horse, human and pig IgG was detected. This antibody may cross react with IgG from other species.
Antiserum was cross adsorbed using bovine, chicken, goat, horse, human and pig immunosorbents to remove cross reactive Antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, human, pig and rabbit IgG was detected. This antibody may cross react with IgG from other species.
Country of Origin
USA

Additional Product Information

Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.

Applications

Not all listed applications have been specifically tested by our laboratory.