Bethyl Laboratories
Mouse anti-Chicken IgY Light Chain Monoclonal Antibody [1Y-263]
Secondary
Catalog #A500-053A
TargetChicken IgY Light Chain
HostMouse
Clone1Y-263
ReactivityChicken
ApplicationICC, IHC, WB
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Product Description
Chicken egg yolk is an alternative source for producing target-specific monoclonal antibodies. The main immunoglobulin present in avian blood (IgY) is transmitted to their offspring and accumulates in egg yolks, which enables the non-invasive harvesting of high amounts of antibodies. The IgY molecule has a structure similar to that of IgG, with two heavy chains, each with a molecular weight of 67 to 70 kDa, and two light chains (L) of 25 kDa.
Specifications
Clonality
Monoclonal
Clone
1Y-263
Host
Mouse
Immunogen
Chicken IgY Light Chain
Isotype
IgG1
Reactivity
Chicken
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
500 µg/ml
Product Information
Applications
ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Monoclonal antibody was purified from cell culture supernatant.
Immunogen was Chicken IgY Light Chain
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Goat anti-Mouse IgG Heavy and Light Chain Antibody (Cat. No. A90-216P).
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Western Blot (WB)1:1,000
Documents
Product DatasheetPDF · A500-053A · Lot 1 (current lot)Open
Product DatasheetPDF · A500-053A · Lot 1 (current lot)Open
Product DatasheetPDF · A500-053A · Lot 1 (current lot)Open
Safety Data SheetPDF · A500-053AOpen
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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